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3 plasmids (pGL1124, pGL1224 and pGL1217) were being produced to allow the alternative of 1 allele of CYC9Lister 427 cells. Trypanosomes had been transfected with Just about every plasmid separately, and then subjected to some second transfection with one of several other plasmids, with a unique resistance gene, with all pairwise combos executed.To